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SFRP2 antibody [C2C3], C-term

Anti-SFRP2 antibody [C2C3], C-term used in Western Blot (WB). GTX111892
Anti-SFRP2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111892
Anti-SFRP2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111892

Cat No. GTX111892

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Pig
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

A431 , HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SFRP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037978

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

secreted frizzled related protein 2 , FRP-2 , SARP1 , SDF-5

Background

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SFRP2 antibody [C2C3], C-term used in Western Blot (WB). GTX111892

GTX111892 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SFRP2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111892

GTX111892 ICC/IF Image

SFRP2 antibody [C2C3], C-term detects SFRP2 protein by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SFRP2 protein stained by SFRP2 antibody [C2C3], C-term (GTX111892) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-SFRP2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111892

GTX111892 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using FRP 2(GTX111892) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169