GeneTex
United States (US)

SFRP4 antibody, Internal

GTX88477_945_IHC-P_18122102_275.jpg
GTX88477_2280_WB_18122102_690.jpg

Cat No. GTX88477

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 2-4μg/ml

Note :

Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland.
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88477-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence TNSSCQCPHILPHQD, from the internal region of the protein sequence according to NP_003005.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FRPHE Antibody , FRP4 Antibody

Cellular Localization

Cytoplasmic in ovarian tumor cells.

Background

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88477_945_IHC-P_18122102_275.jpg

GTX88477 IHC-P Image

IHC-P analysis of human uterus using GTX88477 SFRP4 antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2µg/ml

GTX88477_2280_WB_18122102_690.jpg

GTX88477 WB Image

WB analysis of human uterus lysate using GTX88477 SFRP4 antibody, Internal.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer