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SFRP4 antibody, Internal

Anti-SFRP4 antibody, Internal used in Western Blot (WB). GTX88477
Anti-SFRP4 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88477

Cat. No. GTX88477

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all SFRP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland.
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88477-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence TNSSCQCPHILPHQD, from the internal region of the protein sequence according to NP_003005.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted frizzled related protein 4 , FRP-4 , FRPHE , FRZB-2 , PYL , sFRP-4

Cellular Localization

Secreted

Background

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SFRP4 antibody, Internal used in Western Blot (WB). GTX88477

GTX88477 WB Image

WB analysis of human uterus lysate using GTX88477 SFRP4 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-SFRP4 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88477

GTX88477 IHC-P Image

IHC-P analysis of human uterus using GTX88477 SFRP4 antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399