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SFRP5 antibody

Anti-SFRP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX114747
Anti-SFRP5 antibody used in Western Blot (WB). GTX114747

Cat No. GTX114747

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SFRP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731738

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

secreted frizzled related protein 5 , SARP3

Cellular Localization

Secreted

Background

Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SFRP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX114747

GTX114747 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal colon, using SFRP5(GTX114747) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SFRP5 antibody used in Western Blot (WB). GTX114747

GTX114747 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX114747 diluted at 1:500

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf