GeneTex
United States (US)

SFRP5 antibody

GTX114747_40436_IHC_18121410_753.jpg
GTX114747_40436_WB_18121410_380.jpg

Cat No. GTX114747

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.76mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SFRP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SARP3 antibody, SFRP5 antibody, secreted apoptosis related protein 3 antibody, SARP-3 antibody, sFRP-5 antibody, FRP-1b antibody, frizzled-related protein 1b antibody, secreted frizzled-related protein 5 antibody, secreted apoptosis-related protein 3 antibody

Cellular Localization

Secreted

Background

Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX114747_40436_IHC_18121410_753.jpg

GTX114747 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal colon, using SFRP5(GTX114747) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114747_40436_WB_18121410_380.jpg

GTX114747 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX114747 diluted at 1:500

Reference
SDS
Glycerol.pdf
Thimerosal.pdf