APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:1000 |
1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide, corresponding to amino acids 120-170 of Human SRp20.
Purification
Purified by antigen-affinity chromatography
From serum
Purity
> 95% (by SDS-PAGE)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
serine and arginine rich splicing factor 3 , SFRS3 , SRp20
Cellular Localization
Nucleus,Nucleus speckle,Cytoplasm
Background
The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010]
Database
DATA IMAGES
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GTX66685 IHC-P Image
IHC-P analysis of human liver carcinoma tissue using GTX66685 SFRS3 antibody. Left : Primary antibody Right : Negative control Dilution : 1:50
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GTX66685 WB Image
WB analysis of various samples using GTX66685 SFRS3 antibody. Lane1 : U-87MG whole cell lysate(40ug) Lane2 : Jurkat whole cell lysate(40ug) Lane3 : C6 whole cell lysate(40ug) Lane4 : BV2 whole cell lysate(40ug) Dilution : 1:500
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GTX66685 IHC-P Image
IHC-P analysis of human stomach carcinoma tissue using GTX66685 SFRS3 antibody. Left : Primary antibody Right : Negative control Dilution : 1:50
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REFERENCE
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REVIEW
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