GeneTex
United States (US)

SFRS9 antibody

GTX101465_WB_19011506_548.jpg
GTX101465_39694_IFA_19011506_317.jpg

Cat No. GTX101465

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

293T , A431 , H1299 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.178mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SFRS9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SFRS9 antibody, SRp30c antibody, SRSF9 antibody, pre-mRNA-splicing factor SRp30C antibody, "splicing factor, arginine/serine-rich 9 antibody", SR splicing factor 9 antibody, serine/arginine-rich splicing factor 9 antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two pseudogenes, one on chromosome 15 and the other on chromosome 21, have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX101465_WB_19011506_548.jpg

GTX101465 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: Molt-4 (GTX27912)
C: Raji
12% SDS PAGE
GTX101465 diluted at 1:500

GTX101465_39694_IFA_19011506_317.jpg

GTX101465 ICC/IF Image

SFRS9 antibody detects SFRS9 protein at nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SFRS9 protein stained by SFRS9 antibody (GTX101465) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169