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SGCE antibody [N1C1]

Anti-SGCE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116453
Anti-SGCE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116453
Anti-SGCE antibody [N1C1] used in Western Blot (WB). GTX116453

Cat No. GTX116453

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SGCE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624279

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sarcoglycan epsilon , DYT11 , ESG , epsilon-SG

Cellular Localization

Cell membrane , sarcolemma; Single-pass membrane protein (Potential) , Cytoplasm , cytoskeleton

Background

This gene encodes a member of the sarcoglycan family. Sarcoglycans are transmembrane components in the dystrophin-glycoprotein complex which help stabilize the muscle fiber membranes and link the muscle cytoskeleton to the extracellular matrix. Mutations in this gene have been associated with myoclonus-dystonia syndrome. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-SGCE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116453

GTX116453 IHC-P Image

SGCE antibody [N1C1] detects SGCE protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
SGCE antibody [N1C1] (GTX116453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SGCE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116453

GTX116453 IHC-P Image

SGCE antibody [N1C1] detects SGCE protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
SGCE antibody [N1C1] (GTX116453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SGCE antibody [N1C1] used in Western Blot (WB). GTX116453

GTX116453 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX116453 diluted at 1:1000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169