GeneTex

  • Country / Location Selection

United States (US)

SGK1 antibody

Anti-SGK1 antibody used in Western Blot (WB). GTX03696
Anti-SGK1 antibody used in Flow cytometry (FACS). GTX03696
Anti-SGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03696
Anti-SGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03696
Anti-SGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03696

Cat. No. GTX03696

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Monkey
Package
100 μg ($449)
See all SGK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5μg/ml
ICC/IF 2μg/ml
IHC-P 0.5-1μg/ml
FACS 1-3μg/1x106 cells
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence of human SGK1 (AILIAFMKQRRMGLNDFIQKIAN).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serum/glucocorticoid regulated kinase 1 , SGK

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum membrane , Cell membrane , Mitochondrion

Background

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-SGK1 antibody used in Western Blot (WB). GTX03696

GTX03696 WB Image

WB analysis of various cell lysates using GTX03696 SGK1 antibody.
Dilution : 0.5 μg/mL
Loading : 50 μg per lane

Anti-SGK1 antibody used in Flow cytometry (FACS). GTX03696

GTX03696 FACS Image

FACS analysis of SiHa cells using GTX03696 SGK1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1 μg/1x10⁶ cells

Anti-SGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03696

GTX03696 IHC-P Image

IHC-P analysis of human rectal cancer tissue using GTX03696 SGK1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)

Anti-SGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03696

GTX03696 IHC-P Image

IHC-P analysis of human rectal cancer tissue using GTX03696 SGK1 antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)

Anti-SGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03696

GTX03696 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX03696 SGK1 antibody. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins.
Dilution : 2 μg/mL

REFERENCE

There are currently no references for SGK1 antibody (GTX03696). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for SGK1 antibody (GTX03696). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 449