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SGK1 antibody

Orthogonal
Anti-SGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54726
Anti-SGK1 antibody used in Western Blot (WB). GTX54726
Anti-SGK1 antibody used in Western Blot (WB). GTX54726
Anti-SGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54726
Anti-SGK1 antibody used in Western Blot (WB). GTX54726
Anti-SGK1 antibody used in Western Blot (WB). GTX54726

Cat. No. GTX54726

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SGK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 172-431 of human SGK1 (NP_005618.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887880

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serum/glucocorticoid regulated kinase 1 , SGK

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum membrane,Cell membrane,Mitochondrion

Background

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-SGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54726

GTX54726 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54726 SGK1 antibody.
Dilution : 1:100

Anti-SGK1 antibody used in Western Blot (WB). GTX54726

GTX54726 WB Image

WB analysis of various sample lysates using GTX54726 SGK1 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SGK1 antibody used in Western Blot (WB). GTX54726

GTX54726 WB Image

WB analysis of various sample lysates using GTX54726 SGK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54726

GTX54726 ICC/IF Image

ICC/IF analysis of L929 cells using GTX54726 SGK1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SGK1 antibody used in Western Blot (WB). GTX54726

GTX54726 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2019.PMID: 31744099

Anti-SGK1 antibody used in Western Blot (WB). GTX54726

GTX54726 WB Image

The data was published in the journal PLoS One in 2015. PMID: 25738875

REFERENCE

Application Reference

Garc?a-Arroyo FE et al. Int J Mol Sci 2019; 20 (22) A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose.
Application : WB
Reactivity : Rat
Liao CY et al. Cell Rep 2016; 17 (10) : 2542-2552 Rapamycin Reverses Metabolic Deficits in Lamin A/C-Deficient Mice.
Application : WB
Reactivity : Mouse
Huang AH et al. PLoS One 2015; 10 (3) : e0118530 PARVA promotes metastasis by modulating ILK signalling pathway in lung adenocarcinoma.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399