GeneTex
United States (US)

SGK1 (phospho Ser422) antibody

GTX32413_20190131_WB_19013101_392.jpg
GTX32413_20190131_IHC-P_19013101_674.jpg
GTX32413_20190131_ICCIF_19013101_188.jpg

Cat No. GTX32413

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

49 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of SGK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serum/Glucocorticoid Regulated Kinase 1,Sgk,Sgk1

Cellular Localization

Cell membrane,Cytoplasm,Endoplasmic reticulum membrane,Mitochondrion,Nucleus

Background

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES
GTX32413_20190131_WB_19013101_392.jpg

GTX32413 WB Image

WB analysis of insulin-treated A549 (A), insulin-treated K562 (B) whole cell lysates using GTX32413 SGK1 (phospho Ser422) antibody.

GTX32413_20190131_IHC-P_19013101_674.jpg

GTX32413 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX32413 SGK1 (phospho Ser422) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32413_20190131_ICCIF_19013101_188.jpg

GTX32413 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32413 SGK1 (phospho Ser422) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Package List Price ($)
$ 319