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SGK3 antibody

Anti-SGK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111774
Anti-SGK3 antibody used in Western Blot (WB). GTX111774
Anti-SGK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111774

Cat. No. GTX111774

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SGK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SGK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618413

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serum/glucocorticoid regulated kinase family member 3 , CISK , SGK2 , SGKL

Cellular Localization

Cytoplasmic vesicle , Early endosome

Background

This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SGK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111774

GTX111774 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SGK3(GTX111774) antibody at 1:200 dilution.

Anti-SGK3 antibody used in Western Blot (WB). GTX111774

GTX111774 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX111774 diluted at 1:1000

Anti-SGK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111774

GTX111774 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adrenal gland tumor, using SGK3(GTX111774) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169