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SGLT1 antibody [N3C3]

Anti-SGLT1 antibody [N3C3] used in Western Blot (WB). GTX105367
Anti-SGLT1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105367
Anti-SGLT1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX105367
Anti-SGLT1 antibody [N3C3] used in Western Blot (WB). GTX105367

Cat. No. GTX105367

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Cat, Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SGLT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624670

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 5 member 1 , D22S675 , NAGT , SGLT1

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Glucose transporters are integral membrane proteins that mediate the transport of glucose and structurally-related substances across cellular membranes. Two families of glucose transporter have been identified: the facilitated-diffusion glucose transporter family (GLUT family), also known as 'uniporters,' and the sodium-dependent glucose transporter family (SGLT family), also known as 'cotransporters' or 'symporters' (Wright et al., 1994 [PubMed 7823022]). The SLC5A1 gene encodes a protein that is involved in the active transport of glucose and galactose into eukaryotic and some prokaryotic cells.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SGLT1 antibody [N3C3] used in Western Blot (WB). GTX105367

GTX105367 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] (GTX105367) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SGLT1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105367

GTX105367 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using SGLT1(GTX105367) antibody at 1:500 dilution.

Anti-SGLT1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX105367

GTX105367 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung fibrose tissue, using SGLT1(GTX105367) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SGLT1 antibody [N3C3] used in Western Blot (WB). GTX105367

GTX105367 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] (GTX105367) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

SGLT1 antibody [N3C3] Cat. No. GTX105367

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 08-Dec-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Mouse small intestine tissue
Blocking : Blocking One Histo (Nacalai Tesque), 10min, 25°C
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : Histo VT One (Nacalai Tesque)
Primary Antibody : 1:200, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169