Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] (GTX105367) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded human lung fibrose tissue, using SGLT1(GTX105367) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed A431, using SGLT1(GTX105367) antibody at 1:500 dilution.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SGLT1 antibody [N3C3] (GTX105367) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.