GeneTex
United States (US)

SGOL2 antibody

SGOL2-antibody-GTX86981-IHC-1_18122102_631.jpg

Cat No. GTX86981

Host Rabbit
Clonality Polyclonal
Application IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

We recommend the following starting dilutions:

IHC: 1:50~1:100
ELISA: 1:10000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

144 kDa. ( Note )

Specificity/Sensitivity

SGOL2 Antibody detects endogenous levels of total SGOL2 protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 241% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human SGOL2.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Shugoshin 2,Sgol2,Tripin,Sgo2

Background

Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation (By similarity). Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments.

Database

DATA IMAGES
SGOL2-antibody-GTX86981-IHC-1_18122102_631.jpg

GTX86981 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SGOL2 Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339