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SGSH antibody [C1C3]

Anti-SGSH antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101700
Anti-SGSH antibody [C1C3] used in Western Blot (WB). GTX101700

Cat. No. GTX101700

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SGSH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SGSH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951891

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-sulfoglucosamine sulfohydrolase , HSS , MPS3A , SFMD

Cellular Localization

Lysosome

Background

This gene encodes one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SGSH antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101700

GTX101700 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using SGSH(GTX101700) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SGSH antibody [C1C3] used in Western Blot (WB). GTX101700

GTX101700 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX101700 diluted at 1:5000

REFERENCE

Application Reference

Pavelin J et al. PLoS Pathog 2013; 9 (12) : e1003820 Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169