GeneTex
United States (US)

SH2B antibody

KO/KD
GTX115780_40296_20160307_WB_M_shRNA_watermark_18121410_287.jpg
GTX115780_40296_20170706_WB_R_18121410_117.jpg
GTX115780_40296_20170713_WB_M_R_18121410_638.jpg
GTX115780_40296_IFA_18121410_896.jpg

Cat No. GTX115780

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

C2C12 , PC-12 , Rat-2 , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SH2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sh2B Adaptor Protein 1,Psm,Sh2B,Sh2B1

Cellular Localization

Cytoplasm , Membrane (Probable) , Nucleus

Background

This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]

Database

DATA IMAGES
GTX115780_40296_20160307_WB_M_shRNA_watermark_18121410_287.jpg

GTX115780 WB Image

KO/KD

Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:2000.

GTX115780_40296_20170706_WB_R_18121410_117.jpg

GTX115780 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115780_40296_20170713_WB_M_R_18121410_638.jpg

GTX115780 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115780_40296_IFA_18121410_896.jpg

GTX115780 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using SH2B(GTX115780) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169