Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:2000.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using SH2B(GTX115780) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.