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SH2B antibody

KO/KD
Anti-SH2B antibody used in Western Blot (WB). GTX115780
Anti-SH2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115780
Anti-SH2B antibody used in Western Blot (WB). GTX115780
Anti-SH2B antibody used in Western Blot (WB). GTX115780

Cat. No. GTX115780

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SH2B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

PC-12 , Rat-2 , mouse brain , rat brain , C2C12

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SH2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621972

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH2B adaptor protein 1 , PSM , SH2B

Cellular Localization

Cytoplasm , Membrane (Probable) , Nucleus

Background

This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-SH2B antibody used in Western Blot (WB). GTX115780

GTX115780 WB Image

KO/KD

Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:2000.

Anti-SH2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115780

GTX115780 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using SH2B(GTX115780) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-SH2B antibody used in Western Blot (WB). GTX115780

GTX115780 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SH2B antibody used in Western Blot (WB). GTX115780

GTX115780 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SH2B antibody (GTX115780) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169