APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| Assay dependent |
| 0.3μg/ml |
|
Note :
IHC-P
Human Kidney stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX10103-PEP).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SH3 domain binding protein 1 , ARHGAP43
Cellular Localization
Nucleus , Cytoplasm, cytosol
Background
This gene encodes a member of the Rho GTPase activating protein (RhoGAP) family. The encoded protein regulates Rac signaling and plays a role in cytoskeletal dynamics, cell motility and epithelial junction formation. This protein's association with the exocyst complex, which tethers secretory vesicles to the plasma membrane, has been demonstrated to be important in cell motility. In a distinct complex, this protein has been shown to regulate epithelial junction formation and morphogenesis. By interacting with the Plexin-D1 cell surface receptor, this protein mediates changes in the cytoskeleton in response to semaphorin binding. This protein may promote metastasis in human liver cancer cells and tissues. [provided by RefSeq, Mar 2017]
Database
Research Area
DATA IMAGES
|
GTX10103 WB Image
WB analysis of human brain lysate using GTX10103 SH3BP1 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
|
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|
GTX10103 IHC-P Image
IHC-P analysis of human Kidnay using GTX10103 SH3BP1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 0.3μg/ml
A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney
|
REFERENCE
There are currently no references for SH3BP1 antibody, C-term (GTX10103). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SH3BP1 antibody, C-term (GTX10103). Be the first to share your experience with this product.
