GeneTex
United States (US)

SH3BP1 antibody

GTX107693_39715_IHC_19011506_207.jpg
GTX107693_WB_19011507_637.jpg
GTX107693_39715_IFA_19011507_874.jpg

Cat No. GTX107693

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

A549 , HeLa , Jurkat , NT2D1
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SH3BP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sh3 Domain Binding Protein 1,Arhgap43,Sh3Bp1

Background

Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac.

Database

DATA IMAGES
GTX107693_39715_IHC_19011506_207.jpg

GTX107693 IHC-P Image

SH3BP1 antibody detects SH3BP1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
SH3BP1 antibody (GTX107693) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107693_WB_19011507_637.jpg

GTX107693 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: JurKat
7.5% SDS PAGE
GTX107693 diluted at 1:1000

GTX107693_39715_IFA_19011507_874.jpg

GTX107693 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SH3BP1(GTX107693) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169