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SH3BP1 antibody

Anti-SH3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107693
Anti-SH3BP1 antibody used in Western Blot (WB). GTX107693
Anti-SH3BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107693

Cat No. GTX107693

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

A549 , HeLa , Jurkat , NT2D1

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SH3BP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241326

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SH3 domain binding protein 1 , ARHGAP43

Background

Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac.

Database

DATA IMAGES

Anti-SH3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107693

GTX107693 IHC-P Image

SH3BP1 antibody detects SH3BP1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
SH3BP1 antibody (GTX107693) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SH3BP1 antibody used in Western Blot (WB). GTX107693

GTX107693 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: JurKat
7.5% SDS PAGE
GTX107693 diluted at 1:1000

Anti-SH3BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107693

GTX107693 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SH3BP1(GTX107693) antibody at 1:200 dilution.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169