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SH3BP1 antibody

Anti-SH3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115734
Anti-SH3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115734
Anti-SH3BP1 antibody used in Western Blot (WB). GTX115734

Cat No. GTX115734

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SH3BP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624815

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SH3 domain binding protein 1 , ARHGAP43

Background

Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac.

Database

DATA IMAGES

Anti-SH3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115734

GTX115734 IHC-P Image

SH3BP1 antibody detects SH3BP1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SH3BP1 antibody (GTX115734) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SH3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115734

GTX115734 IHC-P Image

SH3BP1 antibody detects SH3BP1 protein at cytosol on MDA-MB-157 tumor xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-157 tumor xenograft.
SH3BP1 antibody (GTX115734) dilution: 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SH3BP1 antibody used in Western Blot (WB). GTX115734

GTX115734 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX115734 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169