Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody [2F5] (GTX83657) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

FACS analysis of HeLa cells using GTX83657 SH3GL1 antibody [2F5].
Red : Primary antibody
Blue : Negative control antibody

WB analysis of various cell lines using GTX83657 SH3GL1 antibody [2F5].
Loading : 35 ug per lane

WB analysis of wild type and SH3GL1 knock out 293T cell lysate(10μg per lane) using GTX83657 SH3GL1 antibody [2F5].
Dilution : 1:500

ICC/IF analysis of COS7 cells transiently transfected with SH3GL1 plasmid using GTX83657 SH3GL1 antibody [2F5].

IHC-P analysis of human bladder carcinoma tissue using GTX83657 SH3GL1 antibody [2F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

FACS analysis of Jurkat cells using GTX83657 SH3GL1 antibody [2F5].
Red : Primary antibody
Blue : Negative control antibody