GeneTex

  • Country / Location Selection

United States (US)

SH3GL1 antibody [2F5]

KO/KD Orthogonal
Anti-SH3GL1 antibody [2F5] used in Western Blot (WB). GTX83657
Anti-SH3GL1 antibody [2F5] used in Flow cytometry (FACS). GTX83657
Anti-SH3GL1 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83657
Anti-SH3GL1 antibody [2F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83657
Anti-SH3GL1 antibody [2F5] used in Western Blot (WB). GTX83657
Anti-SH3GL1 antibody [2F5] used in Western Blot (WB). GTX83657
Anti-SH3GL1 antibody [2F5] used in Flow cytometry (FACS). GTX83657

Cat. No. GTX83657

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all SH3GL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SH3GL1 (NP_003016) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH3 domain containing GRB2 like 1, endophilin A2 , CNSA1 , EEN , SH3D2B , SH3P8

Cellular Localization

Cytoplasm

Background

Implicated in endocytosis. May recruit other proteins to membranes with high curvature.

Database

Research Area

DATA IMAGES

Anti-SH3GL1 antibody [2F5] used in Western Blot (WB). GTX83657

GTX83657 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody [2F5] (GTX83657) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SH3GL1 antibody [2F5] used in Flow cytometry (FACS). GTX83657

GTX83657 FACS Image

FACS analysis of Jurkat cells using GTX83657 SH3GL1 antibody [2F5].
Red : Primary antibody
Blue : Negative control antibody

Anti-SH3GL1 antibody [2F5] used in IHC (Paraffin sections) (IHC-P). GTX83657

GTX83657 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX83657 SH3GL1 antibody [2F5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SH3GL1 antibody [2F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83657

GTX83657 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SH3GL1 plasmid using GTX83657 SH3GL1 antibody [2F5].

Anti-SH3GL1 antibody [2F5] used in Western Blot (WB). GTX83657

GTX83657 WB Image

KO/KD

WB analysis of wild type and SH3GL1 knock out 293T cell lysate(10μg per lane) using GTX83657 SH3GL1 antibody [2F5].
Dilution : 1:500

Anti-SH3GL1 antibody [2F5] used in Western Blot (WB). GTX83657

GTX83657 WB Image

WB analysis of various cell lines using GTX83657 SH3GL1 antibody [2F5].
Loading : 35 ug per lane

Anti-SH3GL1 antibody [2F5] used in Flow cytometry (FACS). GTX83657

GTX83657 FACS Image

FACS analysis of HeLa cells using GTX83657 SH3GL1 antibody [2F5].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

There are currently no references for SH3GL1 antibody [2F5] (GTX83657). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for SH3GL1 antibody [2F5] (GTX83657). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519