GeneTex

  • Country / Location Selection

United States (US)

SH3GL1 antibody [3F8]

KO/KD Overexpression
Anti-SH3GL1 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX83658
Anti-SH3GL1 antibody [3F8] used in Western Blot (WB). GTX83658
Anti-SH3GL1 antibody [3F8] used in Western Blot (WB). GTX83658
Anti-SH3GL1 antibody [3F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83658
Anti-SH3GL1 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX83658
Anti-SH3GL1 antibody [3F8] used in Western Blot (WB). GTX83658

Cat. No. GTX83658

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($519)
See all SH3GL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SH3GL1 (NP_003016) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH3 domain containing GRB2 like 1, endophilin A2 , CNSA1 , EEN , SH3D2B , SH3P8

Cellular Localization

Cytoplasm

Background

Implicated in endocytosis. May recruit other proteins to membranes with high curvature.

Database

Research Area

DATA IMAGES

Anti-SH3GL1 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX83658

GTX83658 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX83658 SH3GL1 antibody [3F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SH3GL1 antibody [3F8] used in Western Blot (WB). GTX83658

GTX83658 WB Image

Overexpression

WB analysis of HEK293T cells transfected with SH3GL1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83658 SH3GL1 antibody [3F8].
Loading : 5 ug per lane

Anti-SH3GL1 antibody [3F8] used in Western Blot (WB). GTX83658

GTX83658 WB Image

WB analysis of various cell lines using GTX83658 SH3GL1 antibody [3F8].
Loading : 35 ug per lane
Dilution : 1:200

Anti-SH3GL1 antibody [3F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83658

GTX83658 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SH3GL1 plasmid using GTX83658 SH3GL1 antibody [3F8].

Anti-SH3GL1 antibody [3F8] used in IHC (Paraffin sections) (IHC-P). GTX83658

GTX83658 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX83658 SH3GL1 antibody [3F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-SH3GL1 antibody [3F8] used in Western Blot (WB). GTX83658

GTX83658 WB Image

KO/KD

WB analysis of wild type and SH3GL1 knock out 293T cell lysate(10μg per lane) using GTX83658 SH3GL1 antibody [3F8].
Dilution : 1:500

REFERENCE

There are currently no references for SH3GL1 antibody [3F8] (GTX83658). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for SH3GL1 antibody [3F8] (GTX83658). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519