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SH3GL1 antibody [GT3065]

Anti-SH3GL1 antibody [GT3065] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634708
Anti-SH3GL1 antibody [GT3065] used in IHC (Paraffin sections) (IHC-P). GTX634708
Anti-SH3GL1 antibody [GT3065] used in Western Blot (WB). GTX634708

Cat. No. GTX634708

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT3065

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SH3GL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse SH3GL1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888474

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH3-domain GRB2-like 1 , C77078 , EEN , SH3P8 , Sh3d2b

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-SH3GL1 antibody [GT3065] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634708

GTX634708 ICC/IF Image

SH3GL1 antibody [GT3065] detects SH3GL1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SH3GL1 stained by SH3GL1 antibody [GT3065] (GTX634708) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-SH3GL1 antibody [GT3065] used in IHC (Paraffin sections) (IHC-P). GTX634708

GTX634708 IHC-P Image

SH3GL1 antibody [GT3065] detects SH3GL1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
SH3GL1 stained by SH3GL1 antibody [GT3065] (GTX634708) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SH3GL1 antibody [GT3065] used in Western Blot (WB). GTX634708

GTX634708 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody [GT3065] (GTX634708) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations