Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody [GT3065] (GTX634708) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SH3GL1 antibody [GT3065] detects SH3GL1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
SH3GL1 stained by SH3GL1 antibody [GT3065] (GTX634708) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SH3GL1 antibody [GT3065] detects SH3GL1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SH3GL1 stained by SH3GL1 antibody [GT3065] (GTX634708) diluted at 1:500.
Blue: Hoechst 33342 staining.