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SH3GL1 antibody [N3C3]

Orthogonal
Anti-SH3GL1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113548
Anti-SH3GL1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113548
Anti-SH3GL1 antibody [N3C3] used in Western Blot (WB). GTX113548
Anti-SH3GL1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113548

Cat. No. GTX113548

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SH3GL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SH3GL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037981

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH3 domain containing GRB2 like 1, endophilin A2 , CNSA1 , EEN , SH3D2B , SH3P8

Cellular Localization

Cytoplasm , Early endosome membrane; Peripheral membrane protein

Background

This gene encodes a member of the endophilin family of Src homology 3 domain-containing proteins. The encoded protein is involved in endocytosis and may also play a role in the cell cycle. Overexpression of this gene may play a role in leukemogenesis, and the encoded protein has been implicated in acute myeloid leukemia as a fusion partner of the myeloid-lymphoid leukemia protein. Pseudogenes of this gene are located on the long arm of chromosomes 11 and 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SH3GL1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113548

GTX113548 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SH3GL1(GTX113548) antibody at 1:200 dilution.

Anti-SH3GL1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113548

GTX113548 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using EEN(GTX113548) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SH3GL1 antibody [N3C3] used in Western Blot (WB). GTX113548

GTX113548 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody [N3C3] (GTX113548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SH3GL1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113548

GTX113548 ICC/IF Image

Orthogonal

The data was published in the journal Emerg Microbes Infect in 2019.PMID: 31132962

REFERENCE

Application Reference

Chen SL et al. Emerg Microbes Infect 2019; 8 (1) : 773-786 Endophilin-A2-mediated endocytic pathway is critical for enterovirus 71 entry into caco-2 cells.
Application : ICC/IF
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169