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SH3GL1 antibody

Orthogonal
Anti-SH3GL1 antibody used in Western Blot (WB). GTX133333
Anti-SH3GL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133333
Anti-SH3GL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133333
Anti-SH3GL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133333
Anti-SH3GL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133333
Anti-SH3GL1 antibody used in Western Blot (WB). GTX133333

Cat. No. GTX133333

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SH3GL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse SH3GL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886934

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SH3-domain GRB2-like 1 , C77078 , EEN , SH3P8 , Sh3d2b

Cellular Localization

Cytoplasm , Early endosome membrane; Peripheral membrane protein

Background

Implicated in endocytosis. May recruit other proteins to membranes with high curvature.

Database

Research Area

DATA IMAGES

Anti-SH3GL1 antibody used in Western Blot (WB). GTX133333

GTX133333 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody (GTX133333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SH3GL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133333

GTX133333 ICC/IF Image

SH3GL1 antibody detects SH3GL1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SH3GL1 stained by SH3GL1 antibody (GTX133333) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SH3GL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133333

GTX133333 IHC-P Image

SH3GL1 antibody detects SH3GL1 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SH3GL1 antibody (GTX133333) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SH3GL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133333

GTX133333 ICC/IF Image

SH3GL1 antibody detects SH3GL1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SH3GL1 protein stained by SH3GL1 antibody (GTX133333) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SH3GL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133333

GTX133333 IHC-P Image

SH3GL1 antibody detects SH3GL1 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SH3GL1 antibody (GTX133333) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SH3GL1 antibody used in Western Blot (WB). GTX133333

GTX133333 WB Image

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody (GTX133333) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169