Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody (GTX133333) diluted at 1:1000.

SH3GL1 antibody detects SH3GL1 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SH3GL1 antibody (GTX133333) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SH3GL1 antibody detects SH3GL1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SH3GL1 protein stained by SH3GL1 antibody (GTX133333) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SH3GL1 antibody detects SH3GL1 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SH3GL1 antibody (GTX133333) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SH3GL1 antibody detects SH3GL1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SH3GL1 stained by SH3GL1 antibody (GTX133333) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody (GTX133333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.