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SH3GL1 antibody

Orthogonal
Anti-SH3GL1 antibody used in Western Blot (WB). GTX133333
Anti-SH3GL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133333
Anti-SH3GL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133333
Anti-SH3GL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133333
Anti-SH3GL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133333
Anti-SH3GL1 antibody used in Western Blot (WB). GTX133333

Cat. No. GTX133333

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SH3GL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse SH3GL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886934

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH3-domain GRB2-like 1 , C77078 , EEN , SH3P8 , Sh3d2b

Cellular Localization

Cytoplasm , Early endosome membrane; Peripheral membrane protein

Background

Implicated in endocytosis. May recruit other proteins to membranes with high curvature.

Database

Research Area

DATA IMAGES

Anti-SH3GL1 antibody used in Western Blot (WB). GTX133333

GTX133333 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody (GTX133333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SH3GL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133333

GTX133333 ICC/IF Image

SH3GL1 antibody detects SH3GL1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SH3GL1 stained by SH3GL1 antibody (GTX133333) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SH3GL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133333

GTX133333 IHC-P Image

SH3GL1 antibody detects SH3GL1 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SH3GL1 antibody (GTX133333) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SH3GL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133333

GTX133333 ICC/IF Image

SH3GL1 antibody detects SH3GL1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SH3GL1 protein stained by SH3GL1 antibody (GTX133333) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SH3GL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133333

GTX133333 IHC-P Image

SH3GL1 antibody detects SH3GL1 protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SH3GL1 antibody (GTX133333) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SH3GL1 antibody used in Western Blot (WB). GTX133333

GTX133333 WB Image

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL1 antibody (GTX133333) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169