GeneTex
United States (US)

SH3GL2 antibody [N1C1]

GTX106471_40702_20180525_WB_M_R_19011506_395.jpg
GTX106471_40702_20180131_ICC_IF_R_19011506_832.jpg

Cat No. GTX106471

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SH3GL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CNSA2 antibody, EEN-B1 antibody, FLJ20276 antibody, FLJ25015 antibody, SH3D2A antibody, SH3P4 antibody, SH3GL2 antibody, endophilin-A1 antibody, endophilin-1 antibody, Endophilin A1 BAR domain antibody, bA335L15.1 (SH3-domain GRB2-like 2) antibody, SH3 domain-containing GRB2-like protein 2 antibody, SH3 domain protein 2A antibody, SH3-domain GRB2-like 2 antibody

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Database

Research Area

DATA IMAGES
GTX106471_40702_20180525_WB_M_R_19011506_395.jpg

GTX106471 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SH3GL2 antibody [N1C1] (GTX106471) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106471_40702_20180131_ICC_IF_R_19011506_832.jpg

GTX106471 ICC/IF Image

SH3GL2 antibody [N1C1] detects SH3GL2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SH3GL2 stained by SH3GL2 antibody [N1C1] (GTX106471) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169