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SH3GL3 antibody

Anti-SH3GL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114758
Anti-SH3GL3 antibody used in Western Blot (WB). GTX114758
Anti-SH3GL3 antibody used in Western Blot (WB). GTX114758

Cat. No. GTX114758

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SH3GL3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885347

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH3 domain containing GRB2 like 3, endophilin A3 , CNSA3 , EEN-B2 , HsT19371 , SH3D2C , SH3P13

Cellular Localization

Cytoplasm , Early endosome membrane; Peripheral membrane protein

Background

Implicated in endocytosis. May recruit other proteins to membranes with high curvature.

Database

Research Area

DATA IMAGES

Anti-SH3GL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX114758

GTX114758 IHC-P Image

SH3GL3 antibody detects SH3GL3 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SH3GL3 antibody (GTX114758) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SH3GL3 antibody used in Western Blot (WB). GTX114758

GTX114758 WB Image

SH3GL3 antibody detects SH3GL3 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
SH3GL3 antibody (GTX114758) dilution: 1:1000

Anti-SH3GL3 antibody used in Western Blot (WB). GTX114758

GTX114758 WB Image

SH3GL3 antibody detects SH3GL3 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
12 % SDS-PAGE
SH3GL3 antibody (GTX114758) dilution: 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169