GeneTex
United States (US)

SHANK1 antibody [C3], C-term

GTX107701_40114_20171017_IHC-P_M_19011507_481.jpg
GTX107701_40114_IFA_19011507_447.jpg
GTX107701_40114_20150925_IHC-Fr_M_19011507_933.jpg
GTX107701_40114_20170727_IFA_R_19011507_645.jpg
GTX107701_40436_20170831_IHC-Fr_M_19011507_492.jpg
GTX107701_40114_IHC_19011507_613.jpg
GTX107701_40114_20170901_WB_19011507_396.jpg

Cat No. GTX107701

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

225 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.53mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SHANK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SPANK-1 antibody, SSTRIP antibody, synamon antibody, SHANK1 antibody, somatostatin receptor-interacting protein antibody, SH3 and multiple ankyrin repeat domains protein 1 antibody, SSTR-interacting protein antibody, SH3 and multiple ankyrin repeat domains 1 antibody

Background

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
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Database

Research Area

DATA IMAGES
GTX107701_40114_20171017_IHC-P_M_19011507_481.jpg

GTX107701 IHC-P Image

SHANK1 antibody [C3], C-term detects SHANK1 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107701_40114_IFA_19011507_447.jpg

GTX107701 ICC/IF Image

SHANK1 antibody [C3], C-term detects SHANK1 protein at cytoplasm by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX107701_40114_20150925_IHC-Fr_M_19011507_933.jpg

GTX107701 IHC-Fr Image

SHANK1 antibody [C3] detects SHANK1 protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: SHANK1 antibody [C3] (GTX107701) diluted at 1:250. Blue: DAPI

GTX107701_40114_20170727_IFA_R_19011507_645.jpg

GTX107701 ICC/IF Image

SHANK1 antibody [C3], C-term detects SHANK1 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX107701_40436_20170831_IHC-Fr_M_19011507_492.jpg

GTX107701 IHC-Fr Image

SHANK1 antibody [C3], C-term detects SHANK1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: SHANK1 stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX107701_40114_IHC_19011507_613.jpg

GTX107701 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SHANK1(GTX107701) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107701_40114_20170901_WB_19011507_396.jpg

GTX107701 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf