SHANK1 antibody [C3], C-term

Unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data are based on NCBI database.

Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SHANK1 antibody [C3], C-term detects SHANK1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: SHANK1 stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SHANK1 antibody [C3], C-term detects SHANK1 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SHANK1 antibody [C3], C-term detects SHANK1 protein at cytoplasm by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.
Blue: Hoechst 33342 staining.

SHANK1 antibody [C3] detects SHANK1 protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: SHANK1 antibody [C3] (GTX107701) diluted at 1:250. Blue: DAPI

SHANK1 antibody [C3], C-term detects SHANK1 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SHANK1(GTX107701) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min