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SHANK2 antibody

Anti-SHANK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133352
Anti-SHANK2 antibody used in Western Blot (WB). GTX133352
Anti-SHANK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133352

Cat No. GTX133352

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

159 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse SHANK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886949

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SH3 and multiple ankyrin repeat domains 2 , ProSAP1 , mKIAA1022

Cellular Localization

Cytoplasm , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , Cell projection , growth cone

Background

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

Database

Research Area

DATA IMAGES

Anti-SHANK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133352

GTX133352 ICC/IF Image

SHANK2 antibody detects SHANK2 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK2 protein stained by SHANK2 antibody (GTX133352) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SHANK2 antibody used in Western Blot (WB). GTX133352

GTX133352 WB Image

Various tissue extracts (10 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK2 antibody (GTX133352) diluted at 1:1000.

Anti-SHANK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133352

GTX133352 IHC-P Image

SHANK2 antibody detects SHANK2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SHANK2 antibody (GTX133352) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169