GeneTex
United States (US)

SHANK3 antibody

GTX133133_42634_20161013_WB_M_R_18121410_991.jpg
GTX133133_42634_20170727_IFA_R_18121410_906.jpg
GTX133133_42634_20170807_IHC-Fr_M_18121410_476.jpg

Cat No. GTX133133

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

192 kDa. ( Note )

Positive Control

mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse SHANK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sh3 And Multiple Ankyrin Repeat Domains 3,Ai841104,Shank3C-3,Shank3C-4,Shank3B,Shank3

Cellular Localization

Cytoplasm , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density

Background

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

Database

Research Area

DATA IMAGES
GTX133133_42634_20161013_WB_M_R_18121410_991.jpg

GTX133133 WB Image

Various tissue extracts (75 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Shank3 antibody (GTX133133) diluted at 1:1000.

GTX133133_42634_20170727_IFA_R_18121410_906.jpg

GTX133133 ICC/IF Image

SHANK3 antibody detects SHANK3 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK3 protein stained by SHANK3 antibody (GTX133133) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133133_42634_20170807_IHC-Fr_M_18121410_476.jpg

GTX133133 IHC-Fr Image

SHANK3 antibody detects SHANK3 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: SHANK3 protein stained by SHANK3 antibody (GTX133133) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169