GeneTex
United States (US)

SHANK3 antibody

GTX31480_2672_WB_20180221_18121410_578.jpg
GTX31480_1100_IHC-P_20180221_18121410_144.jpg
GTX31480_1099_IHC-P_20180221_18121410_708.jpg

Cat No. GTX31480

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Positive Control

Human Brain Tissue Slide(GTX22203) , 3T3 (NIH) Cell Lysate(GTX27179)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3.The immunogen is located within amino acids 890 - 940 of SHANK3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sh3 And Multiple Ankyrin Repeat Domains 3,Del22Q13.3,Prosap2,Psap2,Sczd15,Spank-2,Shank3

Background

This gene is a member of the Shank gene family. Shank proteins are multidomain scaffold proteins of the postsynaptic density that connect neurotransmitter receptors, ion channels, and other membrane proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Shank proteins also play a role in synapse formation and dendritic spine maturation. Mutations in this gene are a cause of autism spectrum disorder (ASD), which is characterized by impairments in social interaction and communication, and restricted behavioral patterns and interests. Mutations in this gene also cause schizophrenia type 15, and are a major causative factor in the neurological symptoms of 22q13.3 deletion syndrome, which is also known as Phelan-McDermid syndrome. Additional isoforms have been described for this gene but they have not yet been experimentally verified. [provided by RefSeq, Mar 2012]

Research Area

DATA IMAGES
GTX31480_2672_WB_20180221_18121410_578.jpg

GTX31480 WB Image

WB analysis of 3T3 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31480 SHANK3 antibody.
Working concentration : 1 μg/ml

GTX31480_1100_IHC-P_20180221_18121410_144.jpg

GTX31480 IHC-P Image

IHC-P analysis of human brain tissue using GTX31480 SHANK3 antibody.
Working concentration : 20 μg/ml

GTX31480_1099_IHC-P_20180221_18121410_708.jpg

GTX31480 IHC-P Image

IHC-P analysis of human brain tissue using GTX31480 SHANK3 antibody.
Working concentration : 2.5 μg/ml

Package List Price ($)
$ 339