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SHARP1 antibody

Anti-SHARP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04347
Anti-SHARP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04347
Anti-SHARP1 antibody used in Western Blot (WB). GTX04347

Cat. No. GTX04347

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all SHARP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human BHLHE41(Accession Q9C0J9), corresponding to amino acid residues R11-N61.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basic helix-loop-helix family member e41 , BHLHB3 , DEC2 , SHARP1 , hDEC2

Cellular Localization

Nucleus

Background

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-SHARP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04347

GTX04347 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04347 SHARP1 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-SHARP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04347

GTX04347 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX04347 SHARP1 antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-SHARP1 antibody used in Western Blot (WB). GTX04347

GTX04347 WB Image

WB analysis of RAW264.7 whole cell lysates using GTX04347 SHARP1 antibody. The lane on the left was treated with the antigen-specific peptide.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399