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SHARPIN antibody

Anti-SHARPIN antibody used in Western Blot (WB). GTX130231
Anti-SHARPIN antibody used in Immunoprecipitation (IP). GTX130231

Cat. No. GTX130231

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SHARPIN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:800
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

K562 , THP-1

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SHARPIN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886214

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SHANK associated RH domain interactor , SIPL1

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-SHARPIN antibody used in Western Blot (WB). GTX130231

GTX130231 WB Image

SHARPIN antibody detects SHARPIN protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SHARPIN antibody (GTX130231) diluted at a dilution of 1:1000.

Anti-SHARPIN antibody used in Immunoprecipitation (IP). GTX130231

GTX130231 IP Image

Immunoprecipitation of SHARPIN protein from THP-1 whole cell extracts using 5 μg of SHARPIN antibody (GTX130231).
Western blot analysis was performed using SHARPIN antibody (GTX130231) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169