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SHC1 antibody [1D3]

Anti-SHC1 antibody [1D3] used in Western Blot (WB). GTX83655
Anti-SHC1 antibody [1D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83655
Anti-SHC1 antibody [1D3] used in Flow cytometry (FACS). GTX83655
Anti-SHC1 antibody [1D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83655

Cat. No. GTX83655

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D3

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($519)
See all SHC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SHC adaptor protein 1 , SHC , SHCA

Cellular Localization

Cytoplasm

Background

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.

Database

Research Area

DATA IMAGES

Anti-SHC1 antibody [1D3] used in Western Blot (WB). GTX83655

GTX83655 WB Image

WB analysis of HEK293T cells transfected with SHC1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83655 SHC1 antibody [1D3].
Loading : 5 ug per lane

Anti-SHC1 antibody [1D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83655

GTX83655 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SHC1 plasmid using GTX83655 SHC1 antibody [1D3].

Anti-SHC1 antibody [1D3] used in Flow cytometry (FACS). GTX83655

GTX83655 FACS Image

FACS analysis of HEK293T cells transfected with either SHC1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83655 SHC1 antibody [1D3].

Anti-SHC1 antibody [1D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83655

GTX83655 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83655 SHC1 antibody [1D3].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519