Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SHC1 antibody [N3C2], Internal (GTX113267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHC1 antibody [N3C2], Internal (GTX113267) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX113267 diluted at 1:3000