APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
Product Note
This antibody is raised against human SHC1 phosphorylated at Tyr427 of p66Shc (Uniprot #P29353-1). Based on sequence homology, it is corresponding to human SHC1 phosphorylated at Tyr317 of p52Shc (Uniprot #P29353-2).
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Shc1 around the phosphorylation site of tyrosine 427 (P-S-Yp-V-N).
Purification
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
RRID
AB_11173905
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SHC,SHC adaptor protein 1,SHC1,SHCA
Cellular Localization
Cytoplasm,Mitochondrion matrix,Mitochondrion
Background
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
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GTX78947 WB Image
WB analysis of 293 cell lysate using GTX78947 SHC1 (phospho Tyr317) antibody (Lane 3 and 4) and SHC1 antibody (Lane 1 and 2).
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GTX78947 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX78947 SHC1 (phospho Tyr317) antibody.
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REFERENCE
There are currently no references for SHC1 (phospho Tyr317) antibody (GTX78947). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SHC1 (phospho Tyr317) antibody (GTX78947). Be the first to share your experience with this product.
