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SHC1 (phospho Tyr239) antibody

Anti-SHC1 (phospho Tyr239) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50325
Anti-SHC1 (phospho Tyr239) antibody used in Western Blot (WB). GTX50325

Cat. No. GTX50325

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all SHC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Product Note

This antibody is raised against human SHC1 phosphorylated at Tyr349 of p66Shc (Uniprot #P29353-1). Based on sequence homology, it is corresponding to human SHC1 phosphorylated at Tyr239 of p52Shc (Uniprot #P29353-2).

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 349 (H-Q-Y(p)-Y-N) derived from human SHC1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SHC adaptor protein 1 , SHC , SHCA

Cellular Localization

Cytoplasm

Background

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-SHC1 (phospho Tyr239) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50325

GTX50325 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50325 SHC1 (phospho Tyr239) antibody.

Anti-SHC1 (phospho Tyr239) antibody used in Western Blot (WB). GTX50325

GTX50325 WB Image

WB analysis of extracts from HepG2 cells untreated or treated with EGF using GTX50325 SHC1 (phospho Tyr239) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399