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SHC1 (phospho Tyr427) antibody

Anti-SHC1 (phospho Tyr427) antibody used in Western Blot (WB). GTX50326
Anti-SHC1 (phospho Tyr427) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50326
Anti-SHC1 (phospho Tyr427) antibody used in IHC (Paraffin sections) (IHC-P). GTX50326

Cat No. GTX50326

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from human SHC1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SHC adaptor protein 1 , SHC , SHCA

Cellular Localization

Cytoplasm

Background

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-SHC1 (phospho Tyr427) antibody used in Western Blot (WB). GTX50326

GTX50326 WB Image

WB analysis of extracts from 293 cells untreated or treated with PMA using GTX50326 SHC1 (phospho Tyr427) antibody.

Anti-SHC1 (phospho Tyr427) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50326

GTX50326 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50326 SHC1 (phospho Tyr427) antibody.

Anti-SHC1 (phospho Tyr427) antibody used in IHC (Paraffin sections) (IHC-P). GTX50326

GTX50326 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50326 SHC1 (phospho Tyr427) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319