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SHIP1 antibody [GT1287]

Recombinant antibody KO/KD
Anti-SHIP1 antibody [GT1287] used in Western Blot (WB). GTX03199
Anti-SHIP1 antibody [GT1287] used in Western Blot (WB). GTX03199
Anti-SHIP1 antibody [GT1287] used in IHC (Paraffin sections) (IHC-P). GTX03199
Anti-SHIP1 antibody [GT1287] used in Western Blot (WB). GTX03199
Anti-SHIP1 antibody [GT1287] used in IHC (Paraffin sections) (IHC-P). GTX03199
Anti-SHIP1 antibody [GT1287] used in IHC (Paraffin sections) (IHC-P). GTX03199
Anti-SHIP1 antibody [GT1287] used in IHC (Paraffin sections) (IHC-P). GTX03199

Cat. No. GTX03199

3

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1287

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all SHIP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Observed MW

145 kDa. The observed M.W. is based on the following publication. PMID: 32679836, 20147977

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human SHIP.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inositol polyphosphate-5-phosphatase D , SHIP , SHIP-1 , SHIP1 , SIP-145 , hp51CN , p150Ship

Cellular Localization

Cytoplasm , Cell membrane , Cytoplasm, cytoskeleton , Membrane

Background

This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-SHIP1 antibody [GT1287] used in Western Blot (WB). GTX03199

GTX03199 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHIP1 antibody [GT1287] (GTX03199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 32679836, and PMID:20147977

Anti-SHIP1 antibody [GT1287] used in Western Blot (WB). GTX03199

GTX03199 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHIP1 antibody [GT1287] (GTX03199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 32679836, and PMID:20147977

Anti-SHIP1 antibody [GT1287] used in IHC (Paraffin sections) (IHC-P). GTX03199

GTX03199 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX03199 SHIP1 antibody [GT1287].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-SHIP1 antibody [GT1287] used in Western Blot (WB). GTX03199

GTX03199 WB Image

WB analysis of various samples using GTX03199 SHIP1 antibody [GT1287].
Dilution : 1:1000
Loading : 25μg per lane

Anti-SHIP1 antibody [GT1287] used in IHC (Paraffin sections) (IHC-P). GTX03199

GTX03199 IHC-P Image

IHC-P analysis of rat spleen tissue section using GTX03199 SHIP1 antibody [GT1287].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-SHIP1 antibody [GT1287] used in IHC (Paraffin sections) (IHC-P). GTX03199

GTX03199 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX03199 SHIP1 antibody [GT1287].
Dilution : 1:100

Anti-SHIP1 antibody [GT1287] used in IHC (Paraffin sections) (IHC-P). GTX03199

GTX03199 IHC-P Image

IHC-P analysis of mouse spleen tissue section using GTX03199 SHIP1 antibody [GT1287].
Blue : DAPI for nuclear staining
Dilution : 1:100

REFERENCE

There are currently no references for SHIP1 antibody [GT1287] (GTX03199). Be the first to share your publications with this product.
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REVIEW

SHIP1 antibody [GT1287] Cat. No. GTX03199

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-SHIP1 antibody [GT1287] used in Western Blot (WB). GTX03199
Date : Anonymous submitted on 19-Mar-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : iPSC-derived WT and INPP5D KO microglia
Amount used : 10μg
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-SHIP1 antibody [GT1287] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03199
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U-937 WT and INPP5D KO cells
Blocking : PBS, 5% BSA, 5% goat serum, 0.01% Triton X-100, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : PBS, 0.1% Triton X-100, 25°C, 0.17Hr
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : WT cells: Green dashed line KO cells: Magenta dashed line Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-SHIP1 antibody [GT1287] used in Immunoprecipitation (IP). GTX03199
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : IP
Sample Species : Hu
Sample : U-937 cells
Lane Description : SM: 4% starting material UB: 4% unbound fraction
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together, 30 μL of protein A-Sepharose beads. 0.5 mL aliquots at 2.0 mg/mL of lysate were incubated, an antibody-bead conjugate for ~1 hrs at 4ºC. Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
Package List Price ($)
$ 439