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SHIP1 antibody [SHIP-02]

KO/KD
Anti-SHIP1 antibody [SHIP-02] used in Western Blot (WB). GTX79893

Cat. No. GTX79893

2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SHIP-02

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μg ($399)
See all SHIP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11170928

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

INPP5D , SHIP , SHIP1 , SIP145 , hp51CN , inositol polyphosphate5phosphatase D , p150Ship , inositol polyphosphate-5-phosphatase D

Cellular Localization

Cytoplasm,Cell membrane,Membrane raft,Cytoskeleton,Membrane

Background

This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-SHIP1 antibody [SHIP-02] used in Western Blot (WB). GTX79893

GTX79893 WB Image

WB analysis of THP-1 cells using GTX79893 SHIP1 antibody [SHIP-02].
Lane 1 : mouse IgG1 isotype control
Lane 2,3 : GTX79894
Lane 4,5 : GTX79893

REFERENCE

There are currently no references for SHIP1 antibody [SHIP-02] (GTX79893). Be the first to share your publications with this product.
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REVIEW

SHIP1 antibody [SHIP-02] Cat. No. GTX79893

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-SHIP1 antibody [SHIP-02] used in Western Blot (WB). GTX79893
Date : Anonymous submitted on 20-Mar-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : iPSC-derived WT and INPP6D KO microglia
Amount used : 10μg
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-SHIP1 antibody [SHIP-02] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79893
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U-937 WT and INPP5D KO cells
Blocking : PBS, 5% BSA, 5% goat serum, 0.01% Triton X-100, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : PBS, 0.1% Triton X-100, 25°C, 0.17Hr
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : WT cells: Green dashed line KO cells: Magenta dashed line Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399