GeneTex
United States (US)

SHMT2 antibody

GTX125939_40898_IHC_18121410_988.jpg
GTX125939_40898_WB_2_18121410_456.jpg
GTX125939_40898_WB_18121410_822.jpg
GTX125939_41262_IFA_18121410_876.jpg

Cat No. GTX125939

Reference 2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
See all SHMT2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , HeLa , A431 , HepG2

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SHMT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serine Hydroxymethyltransferase 2,Glya,Hel-S-51E,Shmt,Shmt2

Cellular Localization

Mitochondrion

Background

This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX125939_40898_IHC_18121410_988.jpg

GTX125939 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using SHMT2(GTX125939) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX125939_40898_WB_2_18121410_456.jpg

GTX125939 WB Image

SHMT2 antibody detects SHMT2 protein by Western blot analysis.
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
SHMT2 antibody (GTX125939) dilution: 1:5000
GTX125939_40898_WB_18121410_822.jpg

GTX125939 WB Image

Sample (whole cell lysate)
A: 293T 10ug
B: HeLa 10ug
7.5% SDS PAGE
GTX125939 diluted at 1:10000

GTX125939_41262_IFA_18121410_876.jpg

GTX125939 ICC/IF Image

SHMT2 antibody detects SHMT2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 1 sec.
Green: SHMT2 protein stained by SHMT2 antibody (GTX125939) diluted at 1:500.
Blue: Hoechst 33342 staining.
REFERENCE

Application Reference

Lin C et al. Cell Death Dis 2019; 10 (2) : 137 Long noncoding RNA LINC01234 promotes serine hydroxymethyltransferase 2 expression and proliferation by competitively binding miR-642a-5p in colon cancer.
Application : IHC-P
Reactivity : Human
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4) : 744-59 Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer
Application : WB
Reactivity : Human
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169