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SHMT2 antibody

Orthogonal
Anti-SHMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125939
Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX125939
Anti-SHMT2 antibody used in Western Blot (WB). GTX125939
Anti-SHMT2 antibody used in Western Blot (WB). GTX125939
Anti-SHMT2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX125939
Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX125939

Cat. No. GTX125939

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SHMT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , HeLa , A431 , HepG2

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SHMT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174916

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine hydroxymethyltransferase 2 , GLYA , HEL-S-51e , SHMT

Cellular Localization

Mitochondrion

Background

This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SHMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125939

GTX125939 ICC/IF Image

SHMT2 antibody detects SHMT2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 1 sec.
Green: SHMT2 protein stained by SHMT2 antibody (GTX125939) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX125939

GTX125939 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using SHMT2(GTX125939) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SHMT2 antibody used in Western Blot (WB). GTX125939

GTX125939 WB Image

Sample (whole cell lysate)
A: 293T 10ug
B: HeLa 10ug
7.5% SDS PAGE
GTX125939 diluted at 1:10000

Anti-SHMT2 antibody used in Western Blot (WB). GTX125939

GTX125939 WB Image

SHMT2 antibody detects SHMT2 protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
SHMT2 antibody (GTX125939) dilution: 1:5000

Anti-SHMT2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX125939

GTX125939 IHC-Fr Image

Orthogonal

The data was published in the journal Cell Death Dis in 2019. PMID: 30755591

Anti-SHMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX125939

GTX125939 IHC-P Image

The data was published in the journal Cell Death Dis in 2019. PMID: 31395852

REFERENCE

Application Reference

Chu H et al. Anal Chem 2022; 94 (2) : 758-767 Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.
Yan Liao et al. Cancer Cell Int 2021; 21 (1) : 220 Silencing SHMT2 inhibits the progression of tongue squamous cell carcinoma through cell cycle regulation.
Application : WB, IHC-P
Reactivity : Human
Zgheib R et al. Cell Death Dis 2019; 10 (8) : 596 Folate can promote the methionine-dependent reprogramming of glioblastoma cells towards pluripotency.
Application : ICC/IF
Reactivity : Human
Lin C et al. Cell Death Dis 2019; 10 (2) : 137 Long noncoding RNA LINC01234 promotes serine hydroxymethyltransferase 2 expression and proliferation by competitively binding miR-642a-5p in colon cancer.
Application : IHC-P
Reactivity : Human
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4) : 744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169