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SHOC2 antibody

Anti-SHOC2 antibody used in Western Blot (WB). GTX85278
Anti-SHOC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85278
Anti-SHOC2 antibody used in Western Blot (WB). GTX85278

Cat. No. GTX85278

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($419)
See all SHOC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Jurkat Cell Lysate(GTX27899) , Human Spleen Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SHOC2 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of human SHOC2.The immunogen is located within amino acids 30 - 80 of SHOC2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SHOC2 leucine rich repeat scaffold protein , SIAA0862 , SOC2 , SUR8

Cellular Localization

Cytoplasm,Nucleus

Background

SHOC2 protein participates in protein binding / transferase activity in the fibroblast growth factor receptor signaling pathway and Ras protein signal transduction. It is a widely expressed protein composed almost entirely of leucine-rich repeats (LRR), with a lysine-rich sequence at the amino terminus and cytoplasmically localized. SHOC2 acts as a positive modulator of the RAS-MAPK signaling cascade, which is elicited by EGL-15 and LET-23 and mediated by LET-60. SHOC2 together with protein phosphatase 1c (PP1c) forms a highly specific M-Ras effector complex and is essential for activation of the MAPK pathway by growth factors. Furthermore, in tumor cells with Ras gene mutations, inhibition of SHOC2 expression inhibits MAPK, but not PI3K activity. The SHOC2-PP1c holoenzyme provides an attractive therapeutic target for inhibition of the MAPK pathway in cancer. Recent studies show that aberrantly acquired N-myristoylation of SHOC2 causes human disease Noonan-like syndrome with loose anagen hair.

Database

Research Area

DATA IMAGES

Anti-SHOC2 antibody used in Western Blot (WB). GTX85278

GTX85278 WB Image

WB analysis of Jurkate cell lysate using GTX85278 SHOC2 antibody in (A) the absence and (B) the presence of blocking peptide.
Dilution : 1 μg/ml

Anti-SHOC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85278

GTX85278 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85278 SHOC2 antibody.
Working concentration : 5 μg/ml

Anti-SHOC2 antibody used in Western Blot (WB). GTX85278

GTX85278 WB Image

WB analysis of Jurkat cell lysate using GTX85278 SHOC2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419