APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human SHP1
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase non-receptor type 6 , HCP , HCPH , HPTP1C , PTP-1C , SH-PTP1 , SHP-1 , SHP-1L , SHP1
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993](/templates/default/js/lightbox/images/loading.gif "Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993")
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GTX637993 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with SHP1 antibody [HL20845] (GTX637993) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX637993 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP1 antibody [HL20845] (GTX637993) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637993 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP1 antibody [HL20845] (GTX637993) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637993 ICC/IF Image
SHP1 antibody [HL20845] detects SHP1 protein at cytoplasm by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SHP1 stained by SHP1 antibody [HL20845] (GTX637993) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637993 ICC/IF Image
SHP1 antibody [HL20845] detects SHP1 protein at cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SHP1 stained by SHP1 antibody [HL20845] (GTX637993) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for SHP1 antibody [HL20845] (GTX637993). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SHP1 antibody [HL20845] (GTX637993). Be the first to share your experience with this product.
![Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993](/upload/website/prouct_img/small/GTX637993/GTX637993_44970_20230707_WB_competitor_watermark_23071223_813.webp "Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993")
![Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993](/upload/website/prouct_img/small/GTX637993/GTX637993_T-44900_20230106_WB_23010922_534.webp "Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993")
![Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993](/upload/website/prouct_img/small/GTX637993/GTX637993_44970_20230303_WB_TPM_watermark_23030717_704.webp "Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993")
![Anti-SHP1 antibody [HL20845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637993](/upload/website/prouct_img/small/GTX637993/GTX637993_44970_20230428_ICC_IF_23050918_365.webp "Anti-SHP1 antibody [HL20845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637993")
![Anti-SHP1 antibody [HL20845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637993](/upload/website/prouct_img/small/GTX637993/GTX637993_44970_20230526_ICC_IF_23060622_517.webp "Anti-SHP1 antibody [HL20845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637993")
![Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993](/upload/website/prouct_img/normal/GTX637993/GTX637993_44970_20230707_WB_competitor_watermark_23071223_813.webp "Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993")
![Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993](/upload/website/prouct_img/normal/GTX637993/GTX637993_T-44900_20230106_WB_23010922_534.webp "Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993")
![Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993](/upload/website/prouct_img/normal/GTX637993/GTX637993_44970_20230303_WB_TPM_watermark_23030717_704.webp "Anti-SHP1 antibody [HL20845] used in Western Blot (WB). GTX637993")
![Anti-SHP1 antibody [HL20845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637993](/upload/website/prouct_img/normal/GTX637993/GTX637993_44970_20230428_ICC_IF_23050918_365.webp "Anti-SHP1 antibody [HL20845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637993")
![Anti-SHP1 antibody [HL20845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637993](/upload/website/prouct_img/normal/GTX637993/GTX637993_44970_20230526_ICC_IF_23060622_517.webp "Anti-SHP1 antibody [HL20845] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637993")