GeneTex
United States (US)

SHP2 antibody, C-term

GTX29214_170_IHC-P_18121410_592.jpg
GTX29214_1126_WB_18121410_846.jpg

Cat No. GTX29214

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 3-6μg/ml

Note :

IHC-P
Human Cerebellum shows strong cytoplasm staining in Purkinje cells and processes.

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX29214-PEP).

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-YENVGLMQQQKSFR, from the C Terminus of the protein sequence according to NP_002825.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Tyrosine Phosphatase, Non-Receptor Type 11,Bptp3,Cfc,Jmml,Metcds,Ns1,Ptp-1D,Ptp2C,Sh-Ptp2,Sh-Ptp3,Shp2,Ptpn11

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES
GTX29214_170_IHC-P_18121410_592.jpg

GTX29214 IHC-P Image

IHC-P analysis of human cerebellum using GTX29214 SHP2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX29214_1126_WB_18121410_846.jpg

GTX29214 WB Image

WB analysis of human muscle lysate using GTX29214 SHP2 antibody, C-term.
Dilution : 2µg/ml
Loading : 35µg protein in RIPA buffer

REFERENCE
Package List Price ($)
$ 329