APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 3-6μg/ml |
|
Note :
IHC-P
Human Cerebellum shows strong cytoplasm staining in Purkinje cells and processes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX29214-PEP).
Predict Reactivity
Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-YENVGLMQQQKSFR, from the C Terminus of the protein sequence according to NP_002825.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
RRID
AB_384173
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase non-receptor type 11 , BPTP3 , CFC , JMML , METCDS , NS1 , PTP-1D , PTP2C , SH-PTP2 , SH-PTP3 , SHP2
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]
Database
Research Area
DATA IMAGES
|
GTX29214 WB Image
WB analysis of human muscle lysate using GTX29214 SHP2 antibody, C-term.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer
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GTX29214 IHC-P Image
IHC-P analysis of human cerebellum using GTX29214 SHP2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
REVIEW
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