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SHP2 antibody

KO/KD
Anti-SHP2 antibody used in Western Blot (WB). GTX101062
Anti-SHP2 antibody used in Western Blot (WB). GTX101062
Anti-SHP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101062
Anti-SHP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101062
Anti-SHP2 antibody used in Western Blot (WB). GTX101062

Cat. No. GTX101062

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SHP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

rat brain , Mouse brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SHP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951537

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase non-receptor type 11 , BPTP3 , CFC , JMML , METCDS , NS1 , PTP-1D , PTP2C , SH-PTP2 , SH-PTP3 , SHP2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SHP2 antibody used in Western Blot (WB). GTX101062

GTX101062 WB Image

KO/KD

Wild-type (WT) and SHP2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 antibody (GTX101062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SHP2 antibody used in Western Blot (WB). GTX101062

GTX101062 WB Image

SHP2 antibody detects PTPN11 protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
7.5 % SDS-PAGE
SHP2 antibody (GTX101062) dilution: 1:1000

Anti-SHP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101062

GTX101062 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using SHP2(GTX101062) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SHP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101062

GTX101062 IHC-P Image

SHP2 antibody detects PTPN11 protein at cytosol on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
SHP2 antibody (GTX101062) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SHP2 antibody used in Western Blot (WB). GTX101062

GTX101062 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101062 diluted at 1:500

REFERENCE

Application Reference

Mang Y et al. Ann Transl Med 2022; 10 (19) : 1066 Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
Application : WB
Reactivity : Rat
Wang YC et al. Int J Biol Sci 2018; 14 (1) : 47-56 Dioscin overcome TKI resistance in EGFR-mutated lung adenocarcinoma cells via down-regulation of tyrosine phosphatase SHP2 expression.
Application : WB
Reactivity : Human
Washio K et al. Leuk Lymphoma 2017; : 43112 Gene expression analysis of hypersensitivity to mosquito bite, chronic active EBV infection and NK/T-lymphoma/leukemia.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169