GeneTex
United States (US)

SHP2 antibody

KO/KD
GTX101062_40079_20180202_WB_KO_watermark_19011506_716.jpg
GTX101062_WB_19011506_757.jpg
GTX101062_40079_WB_R_brain_19011506_669.jpg
GTX101062_40079_IHC_2_19011506_129.jpg
GTX101062_40079_WB_M_brain_19011506_192.jpg
GTX101062_IHC_19011506_884.jpg

Cat No. GTX101062

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , HeLa , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SHP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Tyrosine Phosphatase, Non-Receptor Type 11,Bptp3,Cfc,Jmml,Metcds,Ns1,Ptp-1D,Ptp2C,Sh-Ptp2,Sh-Ptp3,Shp2,Ptpn11

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101062_40079_20180202_WB_KO_watermark_19011506_716.jpg

GTX101062 WB Image

KO/KD

Wild-type (WT) and SHP2 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 antibody (GTX101062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX101062_WB_19011506_757.jpg

GTX101062 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX101062 diluted at 1:1000

GTX101062_40079_WB_R_brain_19011506_669.jpg

GTX101062 WB Image

SHP2 antibody detects PTPN11 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
SHP2 antibody (GTX101062) dilution: 1:1000

GTX101062_40079_IHC_2_19011506_129.jpg

GTX101062 IHC-P Image

SHP2 antibody detects PTPN11 protein at cytosol on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
SHP2 antibody (GTX101062) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101062_40079_WB_M_brain_19011506_192.jpg

GTX101062 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101062 diluted at 1:500

GTX101062_IHC_19011506_884.jpg

GTX101062 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using SHP2(GTX101062) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169