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SHP2 (phospho Ser576) antibody

Orthogonal
Anti-SHP2 (phospho Ser576) antibody used in Western Blot (WB). GTX17940
Anti-SHP2 (phospho Ser576) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17940

Cat. No. GTX17940

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all SHP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:250
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SHP2 that contains serine 576. The sequence is conserved in mouse, rat and chicken.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase non-receptor type 11 , BPTP3 , CFC , JMML , METCDS , NS1 , PTP-1D , PTP2C , SH-PTP2 , SH-PTP3 , SHP2

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-SHP2 (phospho Ser576) antibody used in Western Blot (WB). GTX17940

GTX17940 WB Image

Orthogonal

WB (peptide competition) analysis of HEK293 cells treated with 100 ng/mL PMA for 15 minutes using GTX17940 SHP2 (phospho Ser576) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), the phosphopeptide corresponding to SHP2 [pS585] (Lane 5), the phosphopeptide corresponding to SHP2 [pS591] (Lane 6), or the phosphopeptide immunogen (Lane 7)control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 1) eliminates the signal further verifying that the antibody is phospho-specific.

Anti-SHP2 (phospho Ser576) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17940

GTX17940 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX17940 SHP2 (phospho Ser576) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399