ICC/IF analysis of NIH-3T3 cells using GTX17940 SHP2 (phospho Ser576) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

WB (peptide competition) analysis of HEK293 cells treated with 100 ng/mL PMA for 15 minutes using GTX17940 SHP2 (phospho Ser576) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), the phosphopeptide corresponding to SHP2 [pS585] (Lane 5), the phosphopeptide corresponding to SHP2 [pS591] (Lane 6), or the phosphopeptide immunogen (Lane 7)control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 1) eliminates the signal further verifying that the antibody is phospho-specific.