WB (peptide competition) analysis of NIH3T3 cells treated with PDGF (Lane 2-5) using GTX17939 SHP2 (phospho Tyr542) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5)control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

IHC-P analysis of human brain tissue using GTX17939 SHP2 (phospho Tyr542) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20