SHP2 (phospho Tyr542) antibody

Cat. No. GTX17939

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	50 μl ($399)

See all SHP2 products

Datasheet
Document

View product citations for antibody GTX17939 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
IHC-P	1:20

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SHP2 that contains tyrosine 542. The sequence is conserved in mouse, rat and chicken.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase non-receptor type 11 , BPTP3 , CFC , JMML , METCDS , NS1 , PTP-1D , PTP2C , SH-PTP2 , SH-PTP3 , SHP2

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]

Database

Gene ID: 5781 PTPN11
UniProt: Q06124 PTPN11

Research Area

DATA IMAGES

GTX17939 IHC-P Image

IHC-P analysis of human brain tissue using GTX17939 SHP2 (phospho Tyr542) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

GTX17939 WB Image

WB (peptide competition) analysis of NIH3T3 cells treated with PDGF (Lane 2-5) using GTX17939 SHP2 (phospho Tyr542) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5)control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

50 μl

$ 399

Qty