Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 (phospho Tyr580) antibody (GTX50329) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 (phospho Tyr580) antibody (GTX50329) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IHC-P analysis of human breast carcinoma tissue using GTX50329 SHP2 (phospho Tyr580) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide