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SIAH1 antibody

KO/KD
Anti-SIAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113268
Anti-SIAH1 antibody used in Western Blot (WB). GTX113268

Cat. No. GTX113268

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, PLA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SIAH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:35705526)

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIAH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618199

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

siah E3 ubiquitin protein ligase 1 , SIAH1A

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SIAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113268

GTX113268 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using SIAH1(GTX113268) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SIAH1 antibody used in Western Blot (WB). GTX113268

GTX113268 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SIAH1 antibody (GTX113268) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Srivastava A et al. Cancer Res 2022; 82 (17) : 3143-3157 MAB21L4 Deficiency Drives Squamous Cell Carcinoma via Activation of RET.
Application : WB, PLA
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169