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SIAH1 antibody

Anti-SIAH1 antibody used in Western Blot (WB). GTX22237
Anti-SIAH1 antibody used in Western Blot (WB). GTX22237

Cat. No. GTX22237

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat, Drosophila
Package
50 μg ($399)
See all SIAH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IP 1:50
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX22237-PEP).

Product Note

This product is expected to recognize both reported isoforms (NP_003022.3; NP_001006611.1)

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3; NP_001006611.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

siah E3 ubiquitin protein ligase 1 , SIAH1A

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SIAH1 antibody used in Western Blot (WB). GTX22237

GTX22237 WB Image

WB analysis of mouse (A, C) and rat (B, D) brain (A, B) and liver (C, D) lysates using GTX22237 SIAH1 antibody.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-SIAH1 antibody used in Western Blot (WB). GTX22237

GTX22237 WB Image

WB analysis of human liver lysate using GTX22237 SIAH1 antibody.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399