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SIAH1 antibody

Anti-SIAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55799
Anti-SIAH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55799
Anti-SIAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55799
Anti-SIAH1 antibody used in Western Blot (WB). GTX55799
Anti-SIAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55799

Cat. No. GTX55799

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SIAH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SIAH1 (NP_001006611.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

siah E3 ubiquitin protein ligase 1 , SIAH1A

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SIAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55799

GTX55799 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX55799 SIAH1 antibody.
Dilution : 1:100

Anti-SIAH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55799

GTX55799 ICC/IF Image

ICC/IF analysis of L929 cells using GTX55799 SIAH1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SIAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55799

GTX55799 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX55799 SIAH1 antibody.
Dilution : 1:100

Anti-SIAH1 antibody used in Western Blot (WB). GTX55799

GTX55799 WB Image

WB analysis of mouse kidney tissue lysate using GTX55799 SIAH1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SIAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55799

GTX55799 IHC-P Image

IHC-P analysis of rat brain tissue using GTX55799 SIAH1 antibody.
Dilution : 1:100

REFERENCE

Application Reference

J Wang et al. J Exp Clin Cancer Res 2023; 42 (1) : 303 Blockage of CacyBP inhibits macrophage recruitment and improves anti-PD-1 therapy in hepatocellular carcinoma
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399