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SIAT4A antibody

Anti-SIAT4A antibody used in IHC (Paraffin sections) (IHC-P). GTX106121
Anti-SIAT4A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106121
Anti-SIAT4A antibody used in Western Blot (WB). GTX106121

Cat No. GTX106121

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIAT4A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952021

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ST3 beta-galactoside alpha-2,3-sialyltransferase 1 , Gal-NAc6S , SIAT4A , SIATFL , ST3GalA , ST3GalA.1 , ST3GalIA , ST3GalIA,1 , ST3O

Cellular Localization

Golgi apparatus , Golgi stack membrane; Single-pass type II membrane protein , Secreted

Background

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SIAT4A antibody used in IHC (Paraffin sections) (IHC-P). GTX106121

GTX106121 IHC-P Image

SIAT4A antibody detects SIAT4A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
SIAT4A stained by SIAT4A antibody (GTX106121) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SIAT4A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106121

GTX106121 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using SIAT4A(GTX106121) antibody at 1:500 dilution.

Anti-SIAT4A antibody used in Western Blot (WB). GTX106121

GTX106121 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SIAT4A antibody (GTX106121) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf