GeneTex
United States (US)

SIGIRR antibody

GTX105412_40786_WB_19011506_310.jpg
GTX105412_40786_IHC_19011506_545.jpg

Cat No. GTX105412

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIGIRR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC110992 antibody, TIR8 antibody, SIGIRR antibody, toll/interleukin-1 receptor 8 antibody, single Ig IL-1-related receptor antibody, single Ig IL-1R-related molecule antibody, single immunoglobulin domain IL1R1 related antibody, single immunoglobulin domain-containing IL1R-related protein antibody, single immunoglobulin and toll-interleukin 1 receptor (TIR) domain antibody

Cellular Localization

Membrane; Single-pass type III membrane protein

Background

Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP.
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Database

DATA IMAGES
GTX105412_40786_WB_19011506_310.jpg

GTX105412 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
10% SDS PAGE
GTX105412 diluted at 1:3000

GTX105412_40786_IHC_19011506_545.jpg

GTX105412 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using SIGIRR(GTX105412) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REVIEW

SIGIRR antibody Cat No. GTX105412

Rating ( Average 3 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 16-May-2014
Score :
Application Tested : IHC-Fr
Sample Species : Hu
Sample : human breast carcinoma tissue
Blocking : 10% FBS, 25°C, 1Hr
Fixation : 4% formalin 15min, then MeOH 3min
Primary Antibody : 1:200, 25°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169