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SIGIRR antibody

Anti-SIGIRR antibody used in Western Blot (WB). GTX105412
Anti-SIGIRR antibody used in IHC (Paraffin sections) (IHC-P). GTX105412

Cat No. GTX105412

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIGIRR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

single Ig and TIR domain containing , IL-1R8 , TIR8

Cellular Localization

Membrane; Single-pass type III membrane protein

Background

Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP.

Database

DATA IMAGES
Anti-SIGIRR antibody used in Western Blot (WB). GTX105412

GTX105412 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
10% SDS PAGE
GTX105412 diluted at 1:3000

Anti-SIGIRR antibody used in IHC (Paraffin sections) (IHC-P). GTX105412

GTX105412 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using SIGIRR(GTX105412) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for SIGIRR antibody (GTX105412). Be the first to share your publications with this product.
REVIEW

SIGIRR antibody Cat No. GTX105412

Rating ( Average 3 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 16-May-2014
Score :
Application Tested : IHC-Fr
Sample Species : Hu
Sample : human breast carcinoma tissue
Blocking : 10% FBS, 25°C, 1Hr
Fixation : 4% formalin 15min, then MeOH 3min
Primary Antibody : 1:200, 25°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169